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Giuseppe Vicidomini

Senior Researcher Tenured - Principal Investigator
Principal Investigator

Research Line

Molecular Microscopy and Spectroscopy


IIT Central Research Labs Genova


Via Morego, 30
+39 010 2897 607
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Giuseppe Vicidomini studied computer science at the Department of Computer and Information Science, University of Genoa (DISI, Genoa, Italy) and received his Diploma in 2003. From 2003 to 2007 he worked at the Laboratory of Advance Microscopy and Spectroscopy (LAMBS, University of Genoa, Genoa, Italy); where he received his Ph.D (advised by Prof. Alberto Diaspro) about image processing and analysis for fluorescence microscopy. From 2008 to 2011 he worked as a post-doctoral fellow at the Department of NonoBiophotonics, Max Planck Institute for Biophysical Chemistry (MPI, Goettingen, Germany); where he developed a new approach to further enhance the resolution ability of stimulated emission deletion (STED) microscopy. Since May 2011 he works in the Department of NanoPhysics, Italian Institute of Technology (IIT, Genoa, Italy), where in September 2013 he obtained a Researcher position and in May 2016 he became principal investigator of the Molecular Microscopy and Spectroscopy research line. His research interests are primarily concentrated on theoretical design, development and validation of novel optical and analytical tools that allow the modern biologists to peer inside living biological systems with unprecedented temporal/spatial abilities and massive information content.

Bibliometric data:

Giuseppe Vicidomini has authored or co-authored 47 articles in peer reviewed international journals (10 as first author, 3 as shared first author, 1 as shared last author, and 12 as last author), 5 reviews in peer reviewed international journals, 15 proceedings in international conferences, 10 chapters in books and 1 editorial. He has given 17 invited talks at international conferences, advanced schools or workshops, 12 oral communications (as speaker) and 2 science cafés. He has filled 4 patents (two granted). 

Bibliometric data (source Scopus, July 2018): total 1476 citations (excluding self-citations), h-index 21.


"Stimulated Emission-Depletion (STED) Microscopy Based on Time Gating of Excitation Beam and Synchronous Detection of Fluorescence Emission," WO/2015/022635

"STED Microscopy with Pulsed Excitation, Continuous Stimulation, and Gated Registration of Spontaneously Emitted Fluorescence Light,” WO/2012/069076 (licensed by Leica Microsystems, PicoQuant, Abberior Instruments)

Popular readings:

G. Vicidomini and G. Moneron, "Gated STED Microscopy with CW-STED Lasers: When the Arrival Time of a Photon Matters," Science Lab 


Super-Resolved Microscopy Time-Resolved Microscopy Single-Molecule


  • Observation of Biomolecular Processes in Live-Cell with Nanocamera, Fondazione San Paolo, Principal Investigator, 2018-2020;
  • AdaptiveSTED, H2020- MSCA-IF-2017, Supervisor (Fellowship applicant Dr Sami Koho), 2019- 2021;
  • BrightEyes, H2020-ERC-CoG-2018, Principal Investigator, 2019-2024

Selected Publications

M. Castello, G. Tortarolo, M. Buttafava, T. Deguchi, F. Villa, S. Koho, L. Pesce, M. Oneto, Pelicci S., L. Lanzanò, P. Bianchini, C.J.R. Sheppard, A. Diaspro, A. Tosi, and G. Vicidomini*, "A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM," Nat. Methods 16(2) (2019) DOI:10.1038/s41592-018-0291-9 (preprint on BioRxiv).

G. Vicidomini*, P. Bianchini, and A. Diaspro, "STED Super-Resolved Microscopy," Nat. Methods 15,173-182(2018) DOI:10.1038/nmeth.4593

G. Tortarolo, M. Castello, A. Diaspro, S. Koho, G. Vicidomini*, "Evaluating Image Resolution in STED Microscopy," Optica 5(1), 32-35 (2018) DOI:10.1364/OPTICA.5.000032

L. Lanzano, L. Scippioni, M. DI Bona, P. Bianchini, R. Bizzarri, F. Cardarelli, A. Diaspro and G. Vicidomini*, "Measurement of nanoscale three-dimensional di usion in the interior of living cells by STED-FCS," Nat. Commun. 8(1), 65 (2017) DOI:10.1038/s41467-017-00117-2

L. Lanzano, I. Coto Hernandez, M. Castello, E. Gratton, A. Diaspro and G. Vicidomini*, "Encoding and decoding spatio-temporal information for super-resolution microscopy", Nat. Commun. 6, 6071 (2015) DOI:10.1038/ncomms7701

G. Vicidomini, G. Moneron, K.Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S.W. Hell, “Sharper low-power STED nanoscopy by time gating”, Nat. Methods 8(7), 571-573 (2011) DOI:10.1038/NMETH.1624


Innovation Awards, SMARTcup Liguria 2016, category "industrial" (with Genoa Instruments)


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