• MiRNA 4 15617
  • FLUO ISH MiR 124 4080 749x388 © 2016 IIT 5124
  • E8 A Copy 4079 749x388 © 2016 IIT 5123
  • MiRNA 5 15618
  • Adult Neurogensis 4077 749x388 © 2016 IIT 5121
  • E14
  • MiRNA 3 15616
  • MiRNA 1 15614
  • Gruppo Davide De Pietri Tonelli 11788

Our research focuses on noncoding RNAs in neurogenesis.

Neurogenesis is the process of new neuron (and glia) generation from Neural Progenitor Cells (NPCs). NPCs self-renew and generate committed offspring in a regulated fashion. The balance between quiescence, proliferation, differentiation and fate decision guarantees brain formation, lifelong neurogenesis and prevents tumour formation. Understanding the molecular control of NPCs fate has implications for comprehending brain malformations and age-related disorders. Over the last three decades, signaling pathways and transcriptional cascades regulating NSCs fate have been elucidated. However, the field has been mostly dominated by a gene-centric view, hence the understanding of NSCs fate remains partial.

MicroRNAs and other noncoding RNAs control NSCs fate by regulating gene expression at the transcriptional and post-transcriptional levels. Current understanding of noncoding RNA functions in NSCs fate is just at the beginning.

Activities

Our research activities are divided into two branches:

  • Brain science: The overarching goal of our research is to understand the role played by microRNAs and other noncoding RNAs in the control of NSCs fate, in mammals.
  • Health: Our aim is to use noncoding RNAs as RNA-based drugs for therapy of Brain cancer and other neural diseases.

Laboratories

Mol.biol. lab; cell cultures facilities (primary neural cells, immortalized lines); induced pluripotent human stem cells lab; histology lab. Access to IIT core facilities: Virus, Nikon center, Animal facility (SPF, individually ventilated cages; Surgery: electroporation, virus injection; behaviour rooms; black cages in collaboration with Tecniplast (Italy)). Deep RNA/DNA seq. and RNAi screening facility.

Collaborations

IIT

  • CGS@SEMM, Milan (F. Nicassio)
  • Non-coding RNAs and RNA-based therapeutics (S. Gustincich)
  • Polymers and Biomaterials (N.Tirelli) 
  • Nanotechnology for Precision Medicine (P. Decuzzi) 

National and International

  • Gerd Kempermann, Director, CRTD and Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Dresden, Germany.
  • Carlos P Fitzsimons Swammerdam Institute for Life Sciences, Univ. of Amsterdam, The Netherlands.
  • Piero Carninci RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.

Industrial collaborations

  • Tecniplast Spa, Buguggiate, VA, Italy.

Projects

  • EU-REA, Marie Curie Actions (MCA) Call: FP7-PEOPLE-2010-RG. TITLE: NEUROGENESIS IN 22Q11.2 DELETION SYNDROME: ROLE OF microRNAs. ACRONYM: MIRNAS/22Q11DS. Project Budget, 100.000 Euros. Project duration 2011-2015.
  • FP7-PEOPLE-2013-IEF. Acronym: “ASTROCLOCK”; Title “Astrocyte regulation of circadian behaviour”. Project Budget: 241.567 €; Project Duration: 2014 – 2016.
  • AIRC - Associazione Italiana per la Ricerca sul Cancro. Title: Stimuli-Responsive Nanoparticles for eradicating different subsets of cancer cells within tumors. Collaborator; Project Budget 180.000 €; Project Duration 02/01/2014-01/01/2017.
  • Fondazione CARIPLO, Call 2015 “Biomedical research on ageing-related diseases”. Title “Role of the astrocyte-mediated circadian clock in neurodegeneration and brain aging”. Co-Prinipal investigator. Project Budget 349,650 €. Project duration 2016-2020
  • AIRC - Investigator Grant. Title: Differentiation therapy for GBM through synergic action of 11 miRNAs/drugs delivered via lipid/polymeric nanoparticles. Project Budget 599.610 €. Project Duration 2018-2022

Patents

  • Process and device for the determination of alterations in neuronal connectivity and/or morphology; 2015-02-19 | patent PAT: US 20150051109 A1
  • Composizione farmaceutica di miRNA e suoi usi terapeutici. Depositato all’ufficio brevetti Italiano Sept.,19th 2016. Rif IIT PT160330; n. 102016000093825